Figure 10.

Effect of fucosterol (Fct) at 5 µM alone and in combination with doxorubicin (Dox) at 0.1, 1, 2, and 5 µM, on cell proliferation in monolayer–72 h (a) and 3D–96 h (b) assessed by BrdU assay. Cells treated with 0.1% DMSO and Dox 5 µM were included as negative and positive controls, respectively. The percentages of cell proliferation are relative to the control and presented as mean + standard deviation of five independent experiments (each in triplicate). (* p < 0.05, ** p < 0.01; *** p < 0.001; **** p < 0.0001).