Table 1.
Antibodies used in this study. Table shows the host species, target area of the protein which the antibody binds and the IHC conditions including stock dilution and the antigen retrieval times (performed in pH6 citrate buffer unless stated).
| Antibody (Reference Number) | Host Species | Binding Area of Protein of Interest | IHC Working Conditions (Antigen Retrieval) |
|---|---|---|---|
| Mcm2 (ab4461) | Rabbit | Aa 1–50 | 1:100 (2 min) |
| PR (RM-9102-s0) | Rabbit | Aa 412–526 | 1:400 (2 min) |
| Stat1 (ab47425) | Rabbit | Aa 694–743 | 1:300 (3 min) |
| Fak (ab40794) | Rabbit | Aa 700–800 | 1:100 (4 min) |
| Slug/Snail (ab180714) |
Rabbit | Aa 236–264 | 1:200 (2 min) |
| Akt (ab179463) | Rabbit | Aa 250 to C terminus | 1:200 (2 min, pH 9 citrate buffer) |