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. 2021 Jan 31;10(2):284. doi: 10.3390/cells10020284

Figure 1.

Figure 1

E-cadherin produces in trans facilitation of early-stage somatic reprogramming. (A) The expression of E-cadherin in different engineered SNL-CDH1-MYC cell lines. Each SNL-CDH1-MYC line was subjected to western blot analysis with anti-E-cadherin and anti-MYC antibodies. (B) Quantitative analysis of the Oct4-GFP-positive colonies during iPSC induction (n = 3). (C) Time-lapse photographs of iPSC colony formation from either γSNL or γSNL-CDH1-MYC feeder cell-seeded MEFCol1a1 4F2A Oct4-GFP cells in iSF medium. Fluorescence microscopy was performed on the 9th to 15th days. The iPS clones in the upper panel were seeded with the parental SNL feeder cells, whereas the lower panel shows clones with the SNL-CDH1-MYC feeder cells. The scale bar denotes 50 µm. *, p < 0.05; **, p < 0.005; ***, p < 0.0005; ns, not significant.