Figure 3.
IL-8 production in PMN upon E. bovis exposure. Supernatants of PMN (1 × 106 per sample; n = 3) treated with TLR2 and TLR4 antibodies and exposed to live E. bovis sporozoites (1:1 ratio; 2 h) were assessed for the presence of IL-8 by ELISA analysis. TLR2-treated PMN exposed to sporozoites showed a significant increase in IL-8 production (* p < 0.05) when compared to PMN in media. Likewise, a significant increase in IL-8 production (** p < 0.01) was observed in the same experimental condition when compared to the respective control without exposure to E. bovis. Data are represented as the mean of 3 replicates ± SD and were analyzed using an unpaired Student’s t-test using GraphPad Prism V.8.4.3 (GraphPad Software Inc., San Diego, CA, USA). p-value notation; * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.