Effect of dietary AX on mitochondrial biogenesis in SO muscle of tail-suspension mice. The total RNA of the SO muscle was extracted and subjected to real-time PCR. Expression ratio relative to that of glycelaldehyde-3-phosphate dehydrogenase (GAPDH). Data are represented as mean ± S.D. (n = 6). Different letters indicate significant differences (p < 0.05) based on the one-way ANOVA and Tukey’s test. Real-time PCR experiments are representative of at least three independent studies. AMPK, adenosine 5′-monophosphate (AMP)-activated protein kinase; PPAR, peroxisome proliferator-activated receptor; Ckmt, creatine kinase in mitochondrial; UCP, uncoupling protein; Atp5g1, ATP synthase, H+ transporting, mitochondrial F0 complex, subunit C1; Ndufaf2, NADH-ubiquinone oxidoreductase complex assembly factor; Sdhb, succinate dehydrogenase complex, subunit B.