Figure 7.

Effect of AX on mitochondrial superoxide levels and MMP in AnA-treated Sol8 myotubes: (a) The rate of superoxide formation in AX-treated Sol8 myotubes was assessed using dihydroethidium (DHE) fluorescence. Succinate and AnA were used as respiratory complex II substrate and complex III inhibitor, respectively; (b) The quantitative matrix metalloproteinase (MMP) values were calculated based on the ratio of fluorescence intensity values (JC-1 dye). Data are represented as mean ± S.D. (n = 8). Different letters indicate significant differences (p < 0.05) based on the one-way ANOVA and Tukey’s test. “−” represents DMSO; *, CCCP; Suc, succinate; AnA, antimycin A. ROS production and MMP levels experiments are representative of at least three independent studies.