Table 2.
List of primers used for the multiplex PCR detection of spa, pvl, and mecA genes.
| Gene | Primer | Sequence (5′–3′) | Size (bp) | Reference |
|---|---|---|---|---|
| mecA |
mecA P4 mecA P7 |
5′-TCCAGATTACAACTTCACCAGG-3′ 5′-CCACTTCATATCTTGTAACG-3′ |
162 | Oliveira and de Lencastre [49] |
| spa * |
spa 113F spa 1514R |
5′-TAAAGACGATCCTTCGGTGAGC-3′ 5′-CAGCAGTAGTGCCGTTTGCTT-3′ |
180–600 | Harmsen et al. [50] |
| pvl |
pvlF pvlR |
5′-GCTGGACAAAACTTCTTGGAATAT-3′ 5′-GATAGGACACCAATAAATTCTGGATTG-3′ |
83 | Deurenberg et al. [51] |
* Its amplicons are of variable sizes (180–600 bp) [52].