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. Author manuscript; available in PMC: 2021 Feb 27.
Published in final edited form as: Placenta. 2019 Nov 25;90:9–17. doi: 10.1016/j.placenta.2019.11.003

Fig. 2. Carbonic anhydrase IX expression in TAPS placentas.

Fig. 2.

A Western blot analysis of placental carbonic anhydrase (CA) IX expression in lysates of polycythemic (P) and anemic (A) territories of 4 representative TAPS placentas. GAPDH served as loading control. B-C. Representative micrographs of polycythemic and anemic placental parenchyma, respectively (hematoxylin-eosin staining, original magnification ×400) D-E. Representative immunohistochemical analysis of corresponding CAIX protein expression in placental parenchyma, showing intense CAIX immunoreactivity, localized to villous trophoblast, stromal and endothelial cells, in the anemic placental share (right), consistent with relative tissue hypoxia. F-G. Representative immunohistochemical analysis of CAIX protein expression in extravillous trophoblast, showing intense membranous immunoreactivity in the anemic placental share (right). (D-G: DAB-peroxidase system with hematoxylin counterstain, original magnification ×400).