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Super-charged natural killer (NK; blue) cell treatment decreased the number of glioblastoma stem cells (GSCs; green) and increased the number of dead cells (PI, red) in 3D tumorsphere models. NK cells were added to GSC tumorspheres at a NK:GSC ratio of 10:1, and images were acquired using an inverted fluorescence microscope 4 h later. Propidium Iodide (PI) staining was used to detect dead cells (red). Scale bars: 100 µm.
