Figure 4.

Visualization of RSV inclusion bodies ex vivo. (A-C) Biopsies of rHRSV-mCherry infected mouse lung were stained to visualize cell membrane and cytoplasm (DiI, red), nucleus (DAPI, cyan) and RSV inclusion bodies (anti-N antibody, green). After clearing, immunostained biopsies were acquired by confocal microscopy. (A) Maximal projection of a 30 µm z-stack showing inclusion bodies in sparse cells of the parenchyma. (B) Representative optical section showing inclusion bodies in the cytoplasm of infected cells. White dotted squares delineate the magnified view presented in (C). (D) Biopsies of rHRSV-mCherry infected mouse lung were stained with anti-P antibody (green) to visualize RSV inclusion bodies. Autofluorescence (red) was acquired to visualize tissues. Representative optical section. White dotted squares delineate the magnified view presented in (E).