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. 2021 Jan 31;10(2):100. doi: 10.3390/biology10020100

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Suppression of transcriptional upregulation of dcl2 by CHV4-C18 p24. Total RNA fractions were obtained from fungal strains shown on the top. C. parasitica C18 non-transformants infected by MyRV2 (C18/MyRV2), and MyRV2 plus CHV4 (C18/MyRV2+CHV4), CHV4 p24 transformants uninfected (C18p24-VF) or infected by MyRV2 (C18p24/MyRV2). Four independent transformants (1 to 5) were tested in this experiment. Total RNA was examined by northern blotting for dcl2 transcripts, MyRV2 S10 mRNA, and CHV4-C18 p24 transgene transcripts. Ribosome RNA (rRNA) serves as a loading control. Mean values of band intensity in the northern blots quantified by ImageJ are shown below each blot. Note that the presence of CHV4 genomic RNA was confirmed in fungal strain C18 doubly infected MyRV2 and CHV4.