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. 2021 Feb 1;9(2):133. doi: 10.3390/biomedicines9020133

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Functional activity of vaccine-induced antibodies as measured by complement-dependent cytotoxicity (CDC) assay. CDC activity was assessed by incubating mouse serum samples with H1N1 virus-infected MDCK cells in the presence of complement. (A) Representative plots of flow cytometric analysis of MDCK cells stained with YO-PRO and Propidium iodide. CDC activity of mouse serum samples was studied three weeks after the second immunization of H7N9, H7N9+4M2e, or PBS (B) or on Day 6 after challenge with SA H1N1 virus (C). Data were compared by one-way ANOVA, followed by Tukey’s multiple comparisons test. * p < 0.05; *** p < 0.001.