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. 2021 Jan 22;10(2):214. doi: 10.3390/cells10020214

Figure 5.

Figure 5

CD24 Expression in ASCs. (A) CD24 gene expression in sorted CD24+ and CD24− ASCs as well as in cultivated ASCs, routinely isolated by the plastic-adherence protocol. Merged data from 4 different donors are shown, gene expression was normalized to RPS18, n = 3 for technical replicates. Mean+SEM is shown, unpaired student´s t-test was performed to calculate significances with *** p < 0.001. (B,C) Intracellular (left panel, permeabilized) and cell-surface (right panel, unpermeabilized) CD24 protein level measured by FACS for (B) sorted CD24− cells and (C) unsorted cultivated ASCs routinely isolated by the plastic-adherence protocol at passage 2. Unstained control (black, dashed lines) and CD24 stained cells (red, solid lines) are shown. (D) CD24 cell-surface expression measured by FACS on ASCs in the course of routine isolation by the plastic-adherence protocol and cultivation. (Left panel) Unsorted SVF cells (red) and plastic-adherent cells after cultivation for 6 days in serum-free ASC1 medium (P-1, light blue) are shown. (Right panel) SVF cells are shown in red. P-1 cells further cultivated for 1 week in serum-containing PM4 medium were referred to as P0 and are shown in dark blue. After freezing, these cells were re-thawed, taken into culture and referred to as ASC passage 1 (P1, orange) and further cultivated to passage 2 (P2, light green) and passage 3 (P3, dark green).