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Thermostability and organic solvents tolerance of Nbs by ELISA based on the antigen 19-NT-OVA and anti-VHH-HRP antibody. (A) anti-19-NT Nbs (1 mg/mL) was incubated at 25, 35, 50, 55, 60, 70, 80 and 90 °C for 5 min. A series concentration (10%, 20%, 40%, 60%, 80% and 100%) of (B) MeOH, (C) acetonitrile.
