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. 2021 Jan 27;9(2):96. doi: 10.3390/vaccines9020096

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Denaturing gel analysis of proteins from planktonic and biofilm cultures of A. baumannii. Two-dimensional electrophoresis gels are shown of cell lysates from cultures harvested in (A,C) planktonic growth phase (P1) or in (B,D) biofilm phase (B1). The gels in Panels A and B were stained with Coomassie Blue to show all visible proteins. Arrows point to several proteins that are present or increased in that gel, but that are absent or decreased in the accompanying gel. Replicate gels were transferred to membranes and probed with polyclonal rat antisera raised against A. baumannii (panels C and D). Panel C shows proteins detected from planktonic growth phase (P1) and Panel D shows proteins detected from biofilm phase (B1). Panels E and F show Western blot analysis of P1, P2, B1, B2, and B3 lysates before and after irradiation in the presence of MDP probed with polyclonal rat sera raised against either planktonic (E) or biofilm (F) forms of A. baumannii AB5075.