Figure 5.

Immunization and intranasal challenge of mice with A. baumannii. (A) Graphic representation of the vaccination schedule. Mice were immunized at weeks 0, 4, and 6. Mice were challenged intranasally at week 8 and monitored daily until the end of week 9. (B–E) Kaplan–Meier Survival Analysis of vaccinated, or mock vaccinated mice challenged with AB5075 via intranasal inoculation as detailed in A. Panels B and C show groups of 8 mice immunized with candidate vaccines as indicated. B Shows survival in a healthy C57BL/6 mouse model, C shows survival in a neutropenic BALB/c model. Panels D and E show a second round of experiments using groups of 10 mice in the healthy C57BL/6 and neutropenic BALB/c models respectively. Log-rank (Mantle–Cox) test results of significance between survival numbers of groups is indicated by ns (not significant, p > 0.05), * p < 0.05, ** p < 0.01, and *** p < 0.001.