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. 2021 Jan 27;9(2):96. doi: 10.3390/vaccines9020096

Table 1.

AB5075 growth conditions and sample designations.

Culture Designation Growth Phase Nutritional Source Time of Harvest Growth Platform
P1 Planktonic TSB Overnight Agitated Broth
P2 Planktonic TSB 2 h Agitated Broth
B1 (Submerged) Biofilm M9 2 days Stationary Broth
B2 (Colony) Biofilm M9 Overnight Stationary Agar
B3 (Colony) Biofilm TSA + SB Overnight Stationary Agar

TSB, tryptic soy broth; M9, M9 minimal media or M9 agar; TSA, trypticase soy agar; SB, sheep red blood. All cultures were grown at 37 °C under standard aerobic conditions.