Table 1.
Culture Designation | Growth Phase | Nutritional Source | Time of Harvest | Growth Platform |
---|---|---|---|---|
P1 | Planktonic | TSB | Overnight | Agitated Broth |
P2 | Planktonic | TSB | 2 h | Agitated Broth |
B1 | (Submerged) Biofilm | M9 | 2 days | Stationary Broth |
B2 | (Colony) Biofilm | M9 | Overnight | Stationary Agar |
B3 | (Colony) Biofilm | TSA + SB | Overnight | Stationary Agar |
TSB, tryptic soy broth; M9, M9 minimal media or M9 agar; TSA, trypticase soy agar; SB, sheep red blood. All cultures were grown at 37 °C under standard aerobic conditions.