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. Author manuscript; available in PMC: 2021 Feb 27.
Published in final edited form as: Nat Metab. 2020 Jul 27;2(8):663–672. doi: 10.1038/s42255-020-0247-0

Fig. 5 ∣. Schematic illustration of the InPOINT pipeline.

Fig. 5 ∣

a, Clone-seq. A massively parallel site-directed-mutagenesis assay to generate individual mutant clones of thousands of missense variants for downstream stability and interaction assays. b, Stability testing. A high-throughput YFP assay to detect mutant (Mut) stability relative to that of the wild-type (WT) protein. HA, haemagglutinin. c, Interactome profiling. A set of five complementary interaction assays to detect changes in specific interactions (loss of known interactions and gain of new interactions) for each mutant. Combining multiple assays ensures data quality and practically eliminates false-positive results.