Figure 4.

Purine complementation in purine mutant, purB::tn of S. aureus, by PA14 in co-culture. Growth of a purine-deficient mutant of S. aureus can be rescued by the wild-type P. aeruginosa cells but not by a P. aeruginosa purine-deficient mutant. The purB::tn mutant growth cannot be recovered when grown with a purine mutant of PA14, suggesting absence of cross feeding between the mutant species. Error bars represent SEM of data derived from five biological replicates on different days, and experiments were performed in technical triplicates each day. Here, ‘***’ designates p < 0.0005 as depicted by two-tailed unpaired Student’s t-test and ns denotes not significant.