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. 2021 Feb 1;10(2):212. doi: 10.3390/antiox10020212

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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The effects of nicotine (NIC), 6-hydroxy-L-nicotine (6HLN), and cotinine (COT) (1 and 2 mg/L) administration in scopolamine (SCOP)-treated zebrafish on acetylcholine esterase (AChE)- (A), superoxide dismutase (SOD)- (B), catalase (CAT)- (C) and glutathione peroxidase (GPX)- (D) specific activities and the content of glutathione (GSH) (E), malondialdehyde (MDA) (F) and carbonylated proteins (G). The values are expressed as means ± S.E.M. (n = 3). ANOVA analysis revealed overall significant differences between the experimental groups for (A) F(7,16) = 11.5, p < 0.0001, (B) F(7,16) = 10.15, p < 0.0001, (C) F(7,16) = 11.31, p < 0.0001, (D) F(7,16) = 14.22, p < 0.0001, (E) F(7,16) = 8.541, p < 0.001, (F) F(7,16) = 12.39, p < 0.0001 and (G) F(7,16) = 14.53, p < 0.0001. For Tukey posthoc analyses—**** p < 0.0001, *** p < 0.001, ** p < 0.01 and * p < 0.05.