Figure 3.

In-vitro cytotoxicity of the fourteen final synthesized compounds on cancerous and non-cancerous cell lines. (A) MDA-MB-231/breast cancer cells; (B) A549/lung adenocarcinoma cells; (C) MDCK/kidney non-cancerous cells. At 1 × 10⁴ cells/well cell density, cells were treated with several drug concentrations (0.1, 1, 10, and 100 µM) of investigated compounds or positive comparator; 5-FU and DOX, for 48 h exposure time. Cytotoxicity was identified via MTT-based colorimetric assay (490 nm) and % cell growth viability was estimated in triplicates as compared to vehicle negative controls representing the untreated cells. Blue dotted line indicates this control.