Figure 7.

Impact of compound 8b on apoptosis rate within MDA-MB-231/breast cancer cell line (Annexin-V/FACS). (A) Apoptotic impact of 8b following cell double staining via Annexin-V-FITC then PI, and then 48 h incubation time with either 8b (13 µM; approximated IC₅₀) or vehicle negative control [0.1% DMSO (v/v)]; (B) Bar-chart representation of quantitative analysis of apoptosis, across different stages, as well as necrosis. The four quadrants are recognised as: LL, LR, UR, and UL for viable, early apoptotic, late apoptotic, and necrotic cell populations, respectively. All data are represented as mean of three independent experiments ± SEM. Statistical analysis was conducted via Student-t-test (**, p < 0.01; ***, p < 0.001).