Figure 9.

Apoptotic gene expressions and protein level analysis of MDA-MB-231/breast cancer cells exposed to 8b. (A) Expression of pro-apoptotic/anti-apoptotic of genes; Bax, Bcl-2, caspase-3, -8, and -9 using RT-PCR method; (B) Evaluation of cytochrome c and class-III β-tubulin protein levels in MDA-MB-231/breast cancer cells using ELISA technique. Within both assays, cultivated MDA-MB-231/breast cancer cells were treated with 8b at its IC₅₀ concentration (13 μM) for 48 h exposure period. All data are represented as average of three independent experiments ± SEM. Statistical analysis was performed using Student-t-test (*, p < 0.05; ***, p < 0.001).