Table 1.

Media composition including supplements used on fresh or cryopreserved tissue.

	Media	Supplements	Type of Tissue
Medium A	100% Cellgro Minimum Essential Medium (MEM) Alpha 1 X (Fisher Scientific) supplemented with 10% foetal bovine serum (Gibco Life Technologies, Rockville, MD, USA) and 1% penicillin-streptomycin–glutamine (29.2 mg/mL L-glutamine, 10,000 units/mL penicillin and 10,000 µg/mL streptomycin sulfate (Gibco® Life Technologies, Rockville, MD, USA) [13]. Plus 0.1% Normocin (Invivogen 500 mg)		Fresh or cryopreserved
Medium B		20 µL/mL ITS (100 µL insulin 10 mg/mL + 100 µL transferrin 5.5 mg/mL + 10 µL selenite 20 µg/mL) (Sigma-Aldrich, Inc, St.Louis, MO, USA)	Fresh or cryopreserved
Medium C		0.1 mM mercaptoethanol (Pharmacia Biotec)	Cryopreserved
Medium D		20 µL/mL ITS 0.1 mM mercaptoethanol (Pharmacia Biotec)	Cryopreserved