Table 2.

Growth patterns of Triturus cristatus cells. Growth patterns of cells from fresh and cryopreserved media in two and four different media, respectively. The numbers shown indicate the number of replicates.

		Lost to Infection		Growth Patterns
	Total no. of replicates	Unsuccessful due to fungus	Unsuccessful due to bacteria	Cell growth not observed	Culture reached 5–100 cells	Cell lines cryopreserved
	Fresh Tissue in Medium A/B
Individual 1	3/3	2/3	0/0	2/0	1/3	0/0
Individual 2	3/3	1/2	0/1	0/1	3/2	1/0
Individual 3	3/3	0/0	0/0	0/0	3/3	0/2
Individual 4	3/3	2/2	0/1	1/1	1/2	0/0
	Cryopreserved Tissue in Medium A/B/C/D
Individual 1	3/3/3/3	1/3/1/1	1/0/0/0	3/3/3/3	0/0/0/0	0/0/0/0
Individual 2	3/3/3/3	0/0/1/1	0/0/0/0	3/1/2/1	0/2/1/2	0/0/0/0
Individual 3	3/3/3/3	2/2/3/1	0/1/2/0	2/3/3/1	1/0/0/2	0/0/0/1
Individual 4	3/3/3/3	2/1/1/2	0/0/0/0	1/3/2/1	2/0/1/2	0/0/0/0

Medias are defined by A, B, C and D, please see Table 1 for more specific information.