Table 3.
Cell index (CI) obtained for different cell numbers of individual 2 at the three time points. Statistical significance was measured between treatments at various cell numbers for treatments A, B and C and at time points (i), (ii) and (iii) using the xCELLigence® system. Values of p < 0.05 are considered to indicate a statistically significant difference.
| Time Point | Cell Number | NoMC(A) (µ ± SE) |
MC(B) (µ ± SE) |
MCPBS(C) (µ ± SE) |
One-Way ANOVA | Turkey’s Test |
|---|---|---|---|---|---|---|
| 20 h (i) | 5000 | 1.60 ± 0.12 | 1.37 ± 0.01 | 1.48 ± 0.13 | F = 2.9 = 1.36, p = 0.30 | |
| 7500 | 3.15 ± 0.13 | 3.06 ± 0.17 | 3.03 ± 0.03 | F = 2.9 = 0.23, p = 0.79 | ||
| 10,000 | 4.45 ± 0.08 | 4.60 ± 0.05 | 4.59 ± 0.05 | F = 2.9 = 1.96, p = 0.19 | ||
| 70 h (ii) | 5000 | 0.85 ± 0.14 | 0.74 ± 0.05 | 0.87 ± 0.09 | F = 2.9 = 0.47, p = 0.63 | |
| 7500 | 2.07 ± 0.23 | 2.10 ± 0.12 | 1.85 ± 0.14 | F = 2.9 = 0.60, p = 0.56 | ||
| 10,000 | 4.47 ± 0.19 | 5.13 ± 0.09 | 4.86 ± 0.17 | F = 2.9 = 4.39, p = 0.04 | (B > A) * | |
| 130 h (iii) | 5000 | 0.48 ± 0.19 | 0.22 ± 0.11 | -0.12 ± 0.04 | F = 2.9 = 5.54, p = 0.02 | (A > C) * |
| 7500 | 1.99 ± 0.47 | 1.34 ± 0.17 | 0.18 ± 0.07 | F = 2.9 = 10.06, p = 0.00 | (A > C) **, (B > C) * | |
| 10,000 | 5.26 ± 0.32 | 6.42 ± 0.23 | 3.72 ± 0.36 | F = 2.9 = 18.89, p = 0.00 | (A > C) *, (B > C) *** |
* = p < 0.05, ** = p < 0.01, *** = p < 0.001. Standard error (SE), Treatment A (No media change (NOMC)), Treatment B (Media change (MC)), Treatment C (Media change with PBS (MCPBS)).