Table 4.
Cell index (CI) obtained for different cell numbers of individual 3 at the three time points. Statistical significance was measured between treatments at various cell numbers for treatments A, B and C and at time points (i), (ii) and (iii) using the xCELLigence® system. Values of p < 0.05 are considered to indicate a statistically significant difference. Standard error (SE).
| Time Point | Cell Number | NoMC(A) (µ ± SE) |
MC (B) (µ ± SE) |
MCPBS (C) (µ ± SE) |
One-Way ANOVA | Turkey’s Test |
|---|---|---|---|---|---|---|
| 20 h (i) | 5000 | 1.61 ± 0.08 | 1.2 ± 0.08 | 1.61 ± 0.06 | F = 2.9 = 1.88, p = 0.20 | |
| 7500 | 2.63 ± 0.13 | 2.16 ± 0.11 | 2.28 ± 0.07 | F = 2.9 = 5.26, p = 0.03 | (A > B) * | |
| 10,000 | 4.90 ± 0.15 | 5.11 ± 0.13 | 4.72 ± 0.08 | F = 2.9 = 2.47, p = 0.13 | ||
| 70 h (ii) | 5000 | 1.84 ± 0.08 | 1.79 ± 0.11 | 1.69 ± 0.05 | F = 2.9 = 0.75, p = 0.49 | |
| 7500 | 6.18 ± 0.15 | 6.08 ± 0.35 | 3.85 ± 0.10 | F = 2.9 = 4.35, p = 0.04 | (A > C) ***, (B > C) *** | |
| 10,000 | 6.45 ± 0.37 | 7.13 ± 0.13 | 6.86 ± 0.11 | F = 2.9 = 2.11, p = 0.17 | ||
| 130 h (iii) | 5000 | 2.21 ± 0.11 | 2.24 ± 0.13 | 1.27 ± 0.03 | F = 2.9 = 30.46, p = 0.00 | (A > C) ***, (B > C) *** |
| 7500 | 3.32 ± 0.12 | 2.81 ± 0.27 | 2.25 ± 0.18 | F = 2.9 = 7.11, p = 0.01 | (A > C) * | |
| 10,000 | 6.19 ± 0.15 | 6.08 ± 0.35 | 3.85 ± 0.10 | F = 2.9 = 33.72, p = 0.00 | (A > C) ***, (B > C) *** |
* = p < 0.05, *** = p < 0.001. Standard error (SE), Treatment A (No media change (NOMC)), Treatment B (Media change (MC)), Treatment C (Media change with PBS (MCPBS)).