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. 2021 Feb 1;10(2):144. doi: 10.3390/antibiotics10020144

Figure 5.

Figure 5

(A) Effect of pH on the enzymatic activity of K64-ORF41 at 25 °C. (B) Effect of different temperatures on the enzymatic activity of K64-ORF41 at pH 7.0. (C) Stability of enzyme activity under different pH values. K64-ORF41 was pre-incubated alone in different pH values for 30 min, and the residual enzyme activity was measured at 25 °C. (D) Stability of enzyme activity under different temperatures. K64-ORF41 was pre-incubated without substrate at various temperatures for 30 min, and the residual enzyme activity was measured at pH 7.0. The results are expressed as the relative activity by comparing with the values under optimal conditions. Data are expressed as means ± SD (n = 3).