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. 2021 Feb 26;22:138. doi: 10.1186/s12864-021-07427-2

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 11 — Analysis of cp sequence characterized amplified region (SCAR) using a 335 bp deletion of the intergenic spacers (trnE/trnT). a By comparing with the cp genomes of three representative diploid C. sinensis species, a 335 bp long deletion was observed in triploid CWN, and the similar long deletion could be found in four other Camellia species, including: C. cuspidate, C. renshanxiangiae, C. elongata and C. gymnogyna. The shadow Blue mean homology = 100%, pink mean homology≥70%, turquoise mean homology≥50% and the dot indicated missing. The number on the right showed the sequence length. The position of the primers was indicated by the arrow. b Only PCR products of CWN had a 335 bp long sequence deletion, while those of other 292 C. sinensis did not. M: D2000 DNA molecular marker; c PCR products of CWN; Lane 1–22: PCR products of 22 examples of randomly selected cultivars from 292 different cultivars covering the majority of C. sinensis cultivars in China. CK: Control. All 292 PCR products were shown in Supplementary Fig. S1. CWN: ‘Wuyi narcissus’ cultivar of C. sinensis var. sinensis (natural triploid Chinary type tea); CSS: C. sinensis var. sinensis (diploid Chinary type tea); CSA: C. sinensis var. assamica (diploid Chinese Assamica type tea); CIA: C. sinensis var. assamica (diploid Indian Assamica type tea)