Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Feb 1;10(2):297. doi: 10.3390/cells10020297

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Selected protein levels in HeLa cells treated with PARP or RNA polymerases’ inhibitors and irradiated with γ-rays. Panels (a,b) The following proteins’ levels were studied: PARP1, γH2AX, lamin A, β-catenin SC-35, SRRM2, and SRSF7. Western blots showed no apoptotic fragmentation of lamin A and PARP1. Quantification of western blot data was performed by ImageJ software. The density of western blot fragments was normalized to the pool of total proteins. Asterisks show increased protein levels. The non-parametric Mann–Whitney test was used for statistical analysis. Asterisks (*) mean α = 0.05. (c) FRAP analysis showed EGFP-SRSF2 recovery kinetics after photobleaching performed in non-treated HeLa cells, and the cell population irradiated with γ-rays, treated with PARPi (olaparib) or affected with α-amanitin. The green asterisk shows the most rapid fluorescence recovery after photobleaching of the EGFP-tagged SRSF2 protein in the cells treated with α-amanitin for 24 h. Data were compared with the control values.