Table 2.

Specification of donors and acceptors used for fluorescence lifetime image microscopy–Förster resonance energy transfer (FLIM-FRET) experiments.

QYCy3 (DONOR)	ECCy5(M−1cm−1)	QYCy5 (ACCEPTOR)	J(λ) (*1 × 1015 M−1cm−1nm4)	R0(Å)	R0 × QYCy5
0.15	250,000	0.30	7.6	49.73	14.92
QYEGFP (DONOR)	EC AF594 * (M−1cm−1)	QYAF594 (ACCEPTOR)	J(λ) (*1 × 1015 M−1cm−1nm4)	R0(Å)	R0 × QYAF594
0.60	92,000	0.66	1.80	49.30	32.54

* AF594 = Alexa Fluor 594. QY = Quantum Yield; EC = Extinction Coefficient; J(λ) = Overlap Integral, $\mathrm{J}\left(\mathsf{\lambda }\right)={\int }_0^{\propto }{\mathrm{F}}_{\mathrm{D}}\left(\mathsf{\lambda }\right){\mathsf{\epsilon }}_{\mathrm{A}}\left(\mathsf{\lambda }\right){\mathsf{\lambda }}^4\mathrm{d}\mathsf{\lambda }/{\int }_0^{\propto }{\mathrm{F}}_{\mathrm{D}}\left(\mathsf{\lambda }\right)\mathrm{d}\mathsf{\lambda }$; R₀ = Föster Radius, $R_0=0.211\sqrt[6]{{\mathsf{\kappa }}^2{\mathrm{n}}^{-4}{\mathrm{Q}}_{\mathrm{D}}\mathrm{J}\left(\mathsf{\lambda }\right)}$; n = refractive index of mounting medium (Vectashield, n = 1.45) and κ² = orientation factor κ² = 0.6667).