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. 2021 Feb 1;10(2):103. doi: 10.3390/biology10020103

Table 2.

Genes and oligonucleotide primers used in this study.

Gene Name Gene Symbol Primer Sequence (5′–3′)
Forkhead box transcription factor a foxa CAGGTATGGGAAGCATGGGA
GCGTATCTCATCGACATGGC
Homeodomain containing transcription factor Hox11/13b hox11/13b TTGCGACGTTCACAACAACT
GTGAGATCGAGAGCCTGTGA
Wint8 wnt8 AAGTGTCATGGCGTCTCTGG
ATGAGCTTGCCACTGACGAA
Deadringer-like dri TGGAAATAGACGAGAGGGGC
GTGGTATCATGGTGGGTGGA
Hex homeodomain transcription factor hex TGAACCACCCTACTCCACTG
GCCGCTCTATTTTGTCCGAG
Spicule matrix protein 50 sm50 GATGGCACACCAGCTTATCC
CTGACGCTTCATGACTGGAG
Biomineralization protein p16 p16 AGCAGGAGCAGTCGGAGATAC
CATCATCACTTCCCATATCGC
Biomineralization protein p19 p19 AGAGACCAGGCAGGAGACTAAG
GTTGATGTCGAGCTTGTCTTTC
Mesenchyme-specific cell surface glycoprotein msp130 ATACATGGCAACCCAAGAAG
CGATTCCAACGAAGATGAGT
Ubiquitin-conjugating enzyme E2 A UBE2a GATTTGAGGAGTGGAGGATTG
AGCTGGCGGATCTTCTTGTA
Histone deacetylase Hdac1 TCACGCCAAGAAGTCAGAAG
CCGTGGTGGATATCAATGTC
Histone acetyltransferase kat2A TGCAATGGATGGAAGAACC
ATGTGCAGCAAGTTGATGG
Histone-lysine N-methyltransferase EHMT2 GTGCAGGAGCTCTTGGTAATG
ACAGAGAGGGTCGGAAAGTG
Histone lysine demethylase phf8 AGCAGTTGCCATTCCTTTTC
CGACCCATTCACATTCCAC
18S ribosomal RNA 18s GAATGTCTGCCCTATCAACTTTCG
TTGGATGTGGTAGCCGTTTCTC