Figure 2.

In vitro activities of CAS (4 µg/mL) in combination with FLC 25 µg/mL; (a), ITC (4 µg/mL; (b), VRC (1 µg/mL; (c), POS 4 µg/mL; (d), AMB 1 µg/mL; (e), and 5FC 0.1 µg/mL; (f) against C. glabrata IFM60089 as determined by the time-killing assay. The detection limit was 20 CFU/mL. Dashed lines represent a >99.9% growth reduction compared with the initial inoculum size for determination of fungicidal activity. CAS-FLC, CAS-VRC, and CAS-POS combinations showed synergetic activities, with only CAS-VRC combination showed fungicidal activity. Each data point represents the means ± standard deviation of results from two independent experiments.