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. 2021 Feb 2;11(2):205. doi: 10.3390/biom11020205

Figure 2.

Figure 2

Deoxycholic acid (DCA) upregulates the expression of endogenous Golgi protein 73 (GP73). (A) The basal expressions of GP73 in Huh-7, SMMC7721, Huh-1, HepAD38, HepG2, and Hep3B cell lines were detected by Western blot (WB). Huh-7 and SMMC7721 cells were treated with 100 μM DCA for 72 h. (B) The cell supernatant level of GP73 was determined by ELISA. (C) Huh-7 and SMMC7721 cells were treated with or without 100 μM DCA for 72 h. The messenger RNA (mRNA) levels of GP73 were determined by qRT-PCR. (D) Huh-7 and SMMC7721 cells were transfected with PGL3 vector containing promoter of GP73 (PGL3-GP73) and treated with or without 100 μM DCA. Samples were harvested 48 h after treatment, and luciferase activities were measured. (E) Huh-7 and SMMC7721 cells were treated with or without 100 μM DCA for 72 h. The protein levels of GP73 were determined by WB. Data in B, C, and D are expressed as the mean ± standard error of the mean (SEM) and represent three independent experiments (* p < 0.05; ** p < 0.01; *** p < 0.001; ns: not significant). Statistical analysis was performed using GraphPad Prism7.