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. 2021 Feb 2;11(2):205. doi: 10.3390/biom11020205

Figure 4.

Figure 4

NF-κB P65 directly binds to the promoter of Golgi protein 73 (GP73). (A) Schematic diagram of chromatin immunoprecipitation (ChIP) PCR (upper). The products of ChIP-PCR in the input, immunoglobulin G (IgG), and ChIP groups were analyzed using agarose gel electrophoresis (lower). (B) Schematic diagram of NF-κB P65 binding site 1 mutant (upper). Huh-7 and SMMC7721 cells were transfected with PGL3-GP73 or GP73-MT and treated with or without 100 μM deoxycholic acid (DCA). Samples were harvested 48 h after treatment, and luciferase activities were measured (lower). Data in B are expressed as the mean ± SEM and represent three independent experiments (* p < 0.05; ** p < 0.001; ns: not significant). Statistical analysis was performed using GraphPad Prism7.