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. 2021 Feb 2;11(2):205. doi: 10.3390/biom11020205

Figure 5.

Figure 5

Deoxycholic acid (DCA) upregulating Golgi protein (GP73) expression through NF-κB pathway in mice. (A) Male C57BL/6 mice were sacrificed at 21 days after being administered phosphate-buffered saline (PBS) or DCA. ALT, AST, and TBA in serum were analyzed. (B) qRT-PCR analysis was carried out to determine GP73 expression level in liver tissues of C57BL/6 mice administered with PBS or DCA for 21 days. (C) GP73 and NF-κB pathway proteins of C57BL/6 mice administered PBS or DCA for 21 days were determined by WB (11 mice for each group). (D) ChIP-PCR assay to determine the binding of NF-kB P65 on GP73 promoter in mouse liver tissues. Three liver tissues from PBS and DCA administration group were randomly selected. The products of ChIP-PCR in the input, IgG, and ChIP groups were analyzed using agarose gel electrophoresis. (E) Serum GP73 levels were determined by ELISA. Data in (A,B,D) are expressed as the mean ± SEM (* p < 0.05; ** p < 0.01; *** p < 0.001; ns: not significant). Statistical analysis was performed using GraphPad Prism7.