Table 2.
Ixodicidal activity on Hyalomma lusitanicum larve (mortality) and antifungal (mycelial growth inhibition) on Fusarium oxysporum, Alternaria alternata, Botrytis cinerea, of the time-course SPH2 extracts.
| Days | H. Lusitanicum | F. Oxysporum | A. Alternata | B. Cinerea |
|---|---|---|---|---|
| LD50 (µg/mg) a | EC50 (mg/mL) b | |||
| 3 | >20 | 0.27 (0.24–0.30) | 0.10 (0.07–0.13) | 0.04 (0.01–0.12) |
| 4 | >20 | 0.25 (0.20–0.31) | 0.14 (0.11–0.18) | 0.05 (0.02–0.10) |
| 6 | >20 | 0.31 (0.26–0.38) | 0.16 (0.12–0.21) | 0.03 (0.02–0.05) |
| 7 | 2.85 (2.61, 3.18) | 0.45 (0.31–0.65) | 0.06 (0.05–0.09) | 0.02 (0.01–0.05) |
| 8 | 7.82 (6.91, 8.75) | 0.31 (0.25–0.39) | 0.25 (0.18–0.34) | 0.12 (0.08–0.18) |
| 10 | 6.63 (5.92–7.49) | 0.21 (0.18–0.25) | 0.16 (0.12–0.22) | 0.16 (0.12–0.22) |
| 12 | 3.30 (3.09, 3.52) | 0.39 (0.31–0.48) | 0.24 (0.20–0.29) | 0.24 (0.20–0.29) |
| 13 | 7.18 (6.67, 7.78) | 0.87 (0.62–1.24) | 0.44 (0.37–0.51) | 0.22 (0.19–0.26) |
a Lethal dose calculated by Probit analysis; b Effective doses calculated by linear regression.