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. 2021 Feb 2;10(2):302. doi: 10.3390/foods10020302

Table 1.

Some control measures for Bacillus cereus spores or vegetative cells.

Control Measure Procedure Treatment/Effect on B. cereus Reference
Control initial microbial load Use of sodium hypochlorite and weak acids on equipment 100 ppm sodium hypochlorite
Weak acids at 30–40 °C for 20–30 min
[34]
Inactivation Heat treatment D-value (90 °C) 3.99–45 min
70 °C for 12 s, 6 log reduction (vegetative cells)
105 °C 36 s, 6 log reduction (spores)
[40,62]
High Hydrostatic Pressure (HPP) More than 1000 MPa [63]
Combined treatments Mild heat and High Hydrostatic Pressure, between 100 and 600 MPa at 30 and 60 °C, 6 log inactivation [64,65]
Olive powder 2.5% and High Hydrostatic Pressure 500 MPa had additive effect [66]
Cold Plasma (CAP) 1.62–2.96 log CFU/mL reductions
Plasma-activated water combined with mild heat, 1.5–2.12 log CFU/g reductions
[67,68,69]
Growth limitation or inhibition Cold storage Below 4 °C [65,70,71]
Carbon dioxide concentration higher than 40% can prevent growth of B. cereus stored at temperature lower than 8 °C [72,73]
Antimicrobials Nisin, 500 IU/g [74]
Enterocin AS-48, 20–35 µg/ml [5]
Chitosan, 2.5% (w/v) [75]
Olive powder, 2.5% (w/v) [76]