Figure 3.

Phylogenetic relationship of nematode GH16 proteins and their relatives from the NCBI database. The branch length of the maximum likelihood tree indicates evolutionary distance. One thousand bootstrap replicates were performed, and the node labels represent the percentage of bootstrap support. The branches with different colored circles correspond to different taxonomic groupings of the species: red = nematodes, green = fungi, and blue = bacteria. A. besseyi GH16-1 and AbGH16-2 is highlighted in bold. The conserved regions and the relative positions of domians in the GH16 protein are shown. Diamond = signal peptide, green rectangle = fungal-specific Lam16A glucanase domain, blue rectangle = laminarinase-like domain, yellow rectangle = other domain(s). The black line at the end of the protein domain schema indicates the relative position of the domains with respect to the C-terminal of the protein.