Figure 7.

Antifungal activity assay of the AbGH16 recombinant protein. (A): The 12% SDS-PAGE analysis of AbGH16 expression in Escherichia coli. Lane M, molecular mass marker; Lane 1, 4, lysate of the non-induced E. coli cells expressing AbGH16-1 and 2; Lane 2, 5, lysate of the IPTG-induced E. coli cells expressing AbGH16-1and 2; Lane 3, 6, purified proteins of AbGH16-1 and AbGH16-2. (B): Germ tube elongation of Botrytis cinerea treated by AbGH16 enzymes. 10 μg purified protein of AbGH16-1 and AbGH16-2 were mixed separately with 1 ml of a B. cinerea spore suspension (3 × 10⁵ spores) in YEPD medium, and incubated at 25 °C for 6 h and 12 h; the same concentration of spore suspension without addition of the proteins was used for a control (3); scale bar = 50 µm. C-D: inhibition of the spore germination rate (C) and germ tube elongation (D) of B. cinerea. The data from two experiments with four replicates per treatment were pooled. Asterisks on the bar indicate significant differences (*, p < 0.05; **, p < 0.01) between treatments as determined by Student’s t-tests.