Table 4.
Univariate analysis and multivariate analysis of the correlation of gene-signature risk score with outcomes among uveal melanoma patients in two cohorts.
| Variable | Univariate Cox Analysis | Multivariate Cox Analysis | ||||||
|---|---|---|---|---|---|---|---|---|
| Coef | HR (95% CI) | z | p-Value | Coef | HR (95% CI) | z | p-Value | |
| TCGA-UVM (overall survival) * | ||||||||
| age | 0.0447 | 1.05 (1.01–1.09) | 2.35 | 0.0186 | 0.101 | 1.11 (0.976–1.25) | 1.57 | 0.115 |
| gender (male vs. female) | 0.433 | 1.54 (0.651–3.65) | 0.984 | 0.325 | ||||
| tumor stage (stage III vs. stage II) | 0.336 | 1.4 (0.556–3.52) | 0.713 | 0.476 | −3.06 | 0.047 (0.00199–1.11) | −1.9 | 0.0579 |
| tumor stage (stage IV vs. stage II) | 4.37 | 79.3 (7.55–834) | 3.64 | 0.000269 | NA | NA | NA | NA |
| extrascleral extension (yes vs. no) | 1.54 | 4.64 (1.5–14.4) | 2.66 | 0.00774 | −4.25 | 0.0142 (3.98× 10−13–5.1× 10 8) | −0.343 | 0.732 |
| tumor diameter | 0.155 | 1.17 (1.01–1.35) | 2.12 | 0.0344 | 0.723 | 2.06 (1.11–3.83) | 2.29 | 0.0221 |
| tumor thickness | 0.111 | 1.12 (0.949–1.32) | 1.33 | 0.183 | ||||
| radiation therapy (yes vs. no) | 1.68 | 5.35 (1.09–26.3) | 2.07 | 0.0389 | 7.79 | 2410 (4.34 × 10−8–1.33 × 10 14) | 0.617 | 0.537 |
| ethnicity (hispanic or latino vs. not hispanic or latino) | −16 | 1.09 × 10−7 (0-Inf) | −0.00205 | 0.998 | ||||
| tissue or organ of origin diagnosis (choroid vs. not choroid) | −0.286 | 0.751 (0.254–2.22) | −0.517 | 0.605 | ||||
| mitotic count | −0.0119 | 0.988 (0.931–1.05) | −0.394 | 0.693 | ||||
| chromosome 3 copy number | −1.86 | 0.156 (0.0574–0.422) | −3.65 | 0.00026 | 2.76 | 15.9 (0.894–281) | 1.88 | 0.0597 |
| chromosome 6p copy number | −1.06 | 0.348 (0.176–0.687) | −3.04 | 0.00237 | −0.874 | 0.417 (0.0559–3.11) | −0.852 | 0.394 |
| chromosome 8q copy number | 0.516 | 1.67 (1.27–2.2) | 3.68 | 0.000235 | −0.88 | 0.415 (0.126–1.37) | −1.44 | 0.149 |
| risk score | 1.65 | 5.22 (2.59–10.5) | 4.61 | 3.99 × 10−6 | 4.23 | 68.6 (3.36–1400) | 2.75 | 0.00598 |
| TCGA-UVM (progression-free survival) # | ||||||||
| age | 0.0271 | 1.03 (0.996–1.06) | 1.7 | 0.0886 | ||||
| gender (male vs. female) | −0.139 | 0.87 (0.422–1.8) | −0.376 | 0.707 | ||||
| tumor stage (stage III vs. stage II) | 0.381 | 1.46 (0.665–3.22) | 0.946 | 0.344 | −0.959 | 0.383 (0.094–1.56) | −1.34 | 0.181 |
| tumor stage (stage IV vs. stage II) | 3.31 | 27.4 (5.06–149) | 3.84 | 0.000124 | 2.87 | 17.6 (0.677–455) | 1.73 | 0.0845 |
| extrascleral extension (yes vs. no) | 1.45 | 4.26 (1.57–11.5) | 2.85 | 0.0044 | −0.682 | 0.506 (0.00917–27.9) | −0.333 | 0.739 |
| tumor diameter | 0.113 | 1.12 (0.999–1.25) | 1.94 | 0.0527 | ||||
| tumor thickness | 0.00859 | 1.01 (0.875–1.16) | 0.119 | 0.905 | ||||
| radiation therapy (yes vs. no) | 0.0846 | 1.09 (0.142–8.33) | 0.0814 | 0.935 | ||||
| ethnicity (hispanic or latino vs. not hispanic or latino) | −17 | 3.98 × 10−8 (0-Inf) | −0.00217 | 0.998 | ||||
| tissue or organ of origin diagnosis (choroid vs. not choroid) | 0.0872 | 1.09 (0.377–3.16) | 0.161 | 0.872 | ||||
| mitotic count | 0.0545 | 1.06 (1.01–1.1) | 2.64 | 0.00829 | 0.0575 | 1.06 (0.976–1.15) | 1.37 | 0.17 |
| chromosome 3 copy number | −1.86 | 0.156 (0.0631–0.386) | −4.02 | 5.88 × 10−5 | −1.3 | 0.272 (0.0293–2.52) | −1.15 | 0.252 |
| chromosome 6p copy number | −0.628 | 0.534 (0.321–0.888) | −2.42 | 0.0155 | 0.709 | 2.03 (0.611–6.75) | 1.16 | 0.247 |
| chromosome 8q copy number | 0.521 | 1.68 (1.31–2.16) | 4.08 | 4.56 × 10−5 | 0.146 | 1.16 (0.546–2.45) | 0.381 | 0.703 |
| risk score | 0.933 | 2.54 (1.65–3.91) | 4.25 | 2.13 × 10−5 | 0.703 | 2.02 (0.486–8.39) | 0.967 | 0.0334 |
| GSE22138 (metastasis-free survival) & | ||||||||
| age | 0.0213 | 1.02 (0.995–1.05) | 1.59 | 0.113 | ||||
| gender (male vs. female) | 0.353 | 1.42 (0.714–2.84) | 1 | 0.316 | ||||
| tumor eye side (left vs. right) | −0.193 | 0.824 (0.424–1.6) | −0.57 | 0.569 | ||||
| tumor location (on equator vs. others) | −0.325 | 0.723 (0.357–1.46) | −0.903 | 0.366 | ||||
| tumor diameter | −0.0165 | 0.984 (0.893–1.08) | −0.336 | 0.737 | ||||
| tumor thickness | 0.116 | 1.12 (0.951–1.33) | 1.37 | 0.171 | ||||
| tumor cell type (epithelioid vs. mixed) | 0.753 | 2.12 (0.954–4.72) | 1.85 | 0.0649 | ||||
| retinal detachment (yes vs. no) | 1.06 | 2.87 (1.24–6.68) | 2.45 | 0.0142 | 0.857 | 2.36 (0.981–5.66) | 1.92 | 0.0553 |
| extrascleral extension (yes vs. no) | 0.563 | 1.76 (0.668–4.62) | 1.14 | 0.253 | ||||
| chromosome 3 status (monosomy vs. disomy) | 1.67 | 5.29 (1.82–15.3) | 3.07 | 0.00217 | 1.24 | 3.45 (1.04–11.5) | 2.02 | 0.0435 |
| risk score | 0.646 | 1.91 (1.31–2.78) | 3.37 | 0.000745 | 0.523 | 1.69 (1.03–2.77) | 2.07 | 0.0388 |
* Multivariate Cox analysis: Concordance = 0.936 (se = 0.049), Likelihood ratio test = 38.13 on 9 df, p = 2 × 10−5, Wald test = 11.72 on 9 df, p = 0.2, Score (logrank) test = 29.7 on 9 df, p = 5 × 10−4; # Multivariate Cox analysis: Concordance = 0.815 (se = 0.067), Likelihood ratio test = 24.64 on 8 df, p = 0.002, Wald test = 19.68 on 8 df, p = 0.01, Score (logrank) test = 47.01 on 8 df, p = 2 × 10−7; & Multivariate Cox analysis: Concordance = 0.764 (se = 0.05), Likelihood ratio test = 20.65 on 3 df, p = 1 × 10−4, Wald test = 17.24 on 3 df, p = 6 × 10−4, Score (logrank) test = 19.56 on 3 df, p = 2 × 10−4; The bold p-value indicates statistical significance.