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. 2021 Feb 3;22(4):1504. doi: 10.3390/ijms22041504

Figure 7.

Figure 7

Effect of ATR inhibition on RT response of HPV-negative and HPV-positive HNSCC xenografts. Relative tumor volume curves of HPV-negative CAL27 and HPV-positive SCC154 xenografts treated with vehicle (10% DMSO/40% propylene glycol) or AZD6738 (50 mg/kg) with(out) RT (5 × 2Gy). Relative tumor growth curves were normalized to the tumor volume at the start of treatment Day 1. Day 1 indicates the start of treatment. Data are presented as mean ± s.e.m, n = 10. * p-value < 0.05 were determined by two-way ANOVA with Bonferroni correction for multiple testing. Western blot analysis of xenograft samples of the HPV-negative CAL27 model (left) and the HPV-positive model SCC154 (right) show the effect of ATR inhibition on CHK1 activation. Tumors for Western blot analysis were harvested at the evening of the last treatment day per schedule. CHK1ser345 (51 kDa), CHK1 (51 kDa), and vinculin (97 kDa) antibodies were used for immunoblotting. N = tumors; kDa = molecular weight as determined by the protein standard.