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. 2021 Feb 2;10(2):224. doi: 10.3390/antiox10020224

Figure 8.

Figure 8

HG activates NADPH via PKC. HREC cells were pre-treated with 2.5 µM CHE for 1 h, before exposure to 5.5 mM glucose (normal glucose, NG) and 30 mM glucose (high glucose, HG) for 10 days. mRNA levels of endothelial (vWF, CD31, CDH5) and mesenchymal markers (VIM, α-SMA, COL1) were determined by qRT-PCR using β-actin for normalization of data (A). Protein level (ng/mL) of endothelial (vWF, CD31, CDH5) and mesenchymal markers (VIM, α-SMA, COL1) were assessed by ELISA (B). Values are shown as mean ± SD, n = 5; *, significantly different from NG; #, significantly different from the HG.