Figure 3.

In vivo CTL activity induced by adjuvant combination vaccine formulations. Mice were immunized with vaccine formulations containing OVA antigen alone or formulated with SLA archaeosomes, other adjuvants (Poly(I:C), R848, CpG, MPLA, QS-21, Pam₃CSK₄) or SLA in combination with other adjuvants. Target cells were formed by pulsing CFSE-labeled splenocytes from naïve mice with CD8 epitopes from OVA and then transferred to immunized mice. On the following day (day 28: 7 days post-2nd immunization), splenocytes were collected and the levels of the target cells determined by flow cytometry. Grouped data are presented as mean + SEM (n = 10/group). ** represents p < 0.01 and **** represents p < 0.0001.