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OVA-specific T cells as determined by ELISpot. Splenocytes of mice immunized with OVA antigen alone or formulated with SLA archaeosomes, other adjuvants (Poly(I:C), R848, CpG, MPLA, QS-21, Pam3CSK4) or SLA in combination with other adjuvants were collected on day 28 (7 days post-2nd immunization) and analyzed by IFN-γ ELISpot when stimulated by CD8 peptide (A) or CD4 peptide (B). Grouped data are presented as mean + SEM (n = 10/group). ** represents p < 0.01.
