Figure 2.

Effect of enzyme/substrate ratio (E/S) on the degree of hydrolysis with Alkalase 2.4 L FG (50 °C, pH 8). (a) RPI as substrate, (b) RAE as substrate, (c) DRM as substrate. Control without enzyme (◆), E/S = 3% (●), E/S = 5% (■). E/S = wet weight of enzyme preparation/dry weight of protein of the substrate. The degree of hydrolysis was measured by the OPA method. Mean ± SD (n = 4). The same letter means there is no significant difference (p < 0.05), 95% confidence level. The comparisons were made among all the observations.