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. 2021 Feb 27;14:49. doi: 10.1186/s13068-021-01899-w

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — Effect of AtFAX1- and AtABCA9-OEs on the expression of genes involved in oil accumulation. Samples were collected with 2-week (upper panel) and 4-week (lower panel) old developing seeds for RNA extraction and real-time PCR. ACT2 (actin2) was used as the internal standard for cDNA input adjustment. The three bars of each genes indicated the three copies of each gene in camelina. Values are the relative expression level compared with WT (red dashed line) and are means ± SE (n = 3). *Significant difference (P < 0.05) based on Student’s t test compared with WT. N/D: not detected. DGAT: Acyl-CoA: diacylglycerol acyltransferase; PDAT: phospholipid: diacylglycerol acyltransferase; WRI1: wrinkled 1; LEC1: leaf cotyledon 1; and NPC6: nonspecific phospholipase C 6