Fig. 4.

Effects of circCTNNA1 silencing and miR-363-3p inhibitor on CRC progression. a The transfection efficiency of in-miR-363-3p was verified by detecting miR-363-3p expression using qRT-PCR in SW620 and SW480 cells. b–l SW620 and SW480 cells were transfected with si-con, si-circCTNNA1#2, si-circCTNNA1#2 + in-miR-con or si-circCTNNA1#2 + in-miR-363-3p. b QRT-PCR was used to measure miR-363-3p expression. CCK8 assay (c, d), colony formation assay (e), flow cytometry (f–h), and transwell assay (i, j) were employed to determine the viability, number of colonies, apoptotic rate, cell cycle, migration and invasion of cells. k, l WB analysis was used to examine the protein expression of E-cadherin and N-cadherin. *p < 0.05, **p < 0.01, ***p < 0.001