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. 2021 Feb 27;22:139. doi: 10.1186/s12864-021-07430-7

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — a. Between group analysis of in vivo and in vitro female and male hatched blastocysts. b. Heatmap displaying the 243 DEGs observed by comparing in vivo produced versus in vitro developed females and the in vivo produced versus in vitro developed males. c. Heatmap displaying genes involved in amino acid metabolism, and d. Significantly enriched canonical pathways between the in vivo developed and in vitro produced hatched blastocysts. Red (−) dots represent canonical pathways of which genes were significantly less expressed in the in vivo embryos, while blue (+) represent canonical pathways of which genes were significantly higher expressed in the in vivo embryos. The GeneRatio indicates the proportion of DEGs that were identified in an enriched canonical pathway