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. 2021 Feb 3;13(2):501. doi: 10.3390/nu13020501

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Long-term DHA supplementation and/or aerobic exercise training regulates the expression of lipid metabolism related genes/proteins in liver of 18-month-old DIO mice. (A–H) mRNA expression levels of genes involved in hepatic lipogenesis (A–D), lipolysis (E) and fatty acid oxidation (F–H). (I) Representative Western blot and densitometry analysis of AMPK phosphorylated and total (left panel). Band densities of phosphorylated AMPK were normalized by total AMPK (right panel). Data are expressed as mean ± SEM. n = 6–10. * p < 0.05, ** p < 0.01, *** p < 0.001 vs. C group. ^# p < 0.05, ^### p < 0.001 vs. DIO group. ^††† p < 0.001 for DIO + DHA vs. DIO + DHA + EX group. ^a p < 0.05, ^b p < 0.01, ^c p < 0.001 for DIO + EX vs. DIO + DHA + EX group.